The Accuri C6 is based in the medical school as is our most smallest analyser. With 2 lasers: 488 blue and 635 Red, and 4 parameters. The Accuri has no adjustable PMT so there are no voltages to set, making it very straight forward to use.
Jun 29, 2017 Valve issues, fluidics leaking onto electronics, flow cell issues, laser problems, etc. We have about 50k to spend on a new flow cytometer. BD has
Facilitates standard three detectors off blue laser, and one off red laser. Allows for four blue detectors, or two blue and two red detectors. Regulatory Status RUO BD has discontinued selling the BD Accuri™ C6 flow cytometer as of December 31, 2016. Service for BD Accuri C6 systems and their related options will continue until April 15, 2021.
The result is that each BD Accuri C6 cytometer is manufactured with standardized fluorescence performance so that users do not need to adjust detector voltages. 1.3 BD Accuri C6 Flow Cytometer Specifications Table 1-3. BD Accuri C6 Flow Cytometer Specifications. Laser Excitation.
Class 1 Laser Product. For Research Use Only.
The BD Accuri ® C6 is a digital basic flow cytometer, which is equipped with a Blue (488nm) and a Red (640nm) lasers, two light scatter detectors (FSC and SSC), and four fluorescence detectors. The default configuration of the BD Accuri ® C6 is similar to the FACSCalibur-2 (3-Blue 1-Red) with filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC.
5Standard filter in alternate position. BD Accuri C6 Flow Cytometer Instrument Manual 2 7820018-01 Rev-2 Table 1-3. BD Accuri C6 Flow Cytometer Specifications Laser Excitation 488 nm (rated at 20,000 hr life) 640 nm (rated at 20,000 hr life) Laser Profile 10 x 75 µm Laser Power 14.7 mW 640 nm Diode Red Laser 20 mW 488 nm Solid State Blue Laser The BD Accuri ® C6 is a digital basic flow cytometer, which is equipped with a Blue (488nm) and a Red (640nm) lasers, two light scatter detectors (FSC and SSC), and four fluorescence detectors. The default configuration of the BD Accuri ® C6 is similar to the FACSCalibur-2 (3-Blue 1-Red) with filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. 2.5.
användning av en flödescytometer (BD Accuri ™ C6; BD Biosciences, USA). GFP-fluorescens exciterades av en 488 nm laser och övervakades genom ett FL1
Training can be requested via PPMS. Access to training videos presented by FCR staff are here. C6 Configuration: The C6 contains two collinear lasers and is arranged in such a way that four detectors all … Small and easily transportable, the BD Accuri C6 Plus cytometer measures 11 x 14.75 x 16.5 in.
The default configuration of the BD Accuri ® C6 is similar to the FACSCalibur-2 (3-Blue 1-Red) with filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC.
Troubleshooting for BYU Life Sciences' Accuri C6 Flow CytometerNarrated by Dr. Donald BreakwellFilmed and edited by Gabriel Goulding
The BD Accuri™ C6 Plus personal flow cytometer is the newest generation of the BD Accuri™ platform. Enhanced sensitivity, reliability, and capabilities bring
Optional filters and the Selectable Lasers Module expand the available fluorochrome combinations. During manufacture, laser and optical alignments are set and locked down. The result is that each BD Accuri® C6 flow cytometer is manufactured with standardized fluorescence performance so that users do not need to adjust detector voltages. The BD Accuri C6 flow cytometer is a small benchtop anlyser equipped with 2 lasers, blue (488nm) and red (633nm) and is capable of 4 colour, 6 parameter analysis (4 colour + FSC + SSC). The Accuri C6 has a fixed optical alignment and pre-optmized detector settings and data is collected over a 7 decade dynamic range, therefore adjustment of detector voltages is not necessary. Suggested Compensation Values for the BD Accuri C6. Compensation Controls – Beads Advantages of compensation beads – Easy to use – Do not require cells 29 FITC PE. BD Accuri™ C6 Software Sample Grid Cytometer Status Standard C6 configuration Selectable Lasers: 2-blue 2-red.
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BD Accuri C6 Flow Cytometer Specifications Laser Excitation 488 nm (rated at 20,000 hr life) 640 nm (rated at 20,000 hr life) Laser Profile 10 x 75 µm Laser Power 14.7 mW 640 nm Diode Red Laser 20 mW 488 nm Solid State Blue Laser The BD Accuri ® C6 is a digital basic flow cytometer, which is equipped with a Blue (488nm) and a Red (640nm) lasers, two light scatter detectors (FSC and SSC), and four fluorescence detectors. The default configuration of the BD Accuri ® C6 is similar to the FACSCalibur-2 (3-Blue 1-Red) with filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. 2.5. Laser Precautions The C6 Flow Cytometer contains a solid state 488nm and a 640nm diode laser under the outer and secondary covers.
Accuri C6 is a digital PC based bench top analyser, 2 laser system.
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1.3 BD Accuri C6 Flow Cytometer Specifications Table 1-3. BD Accuri C6 Flow Cytometer Specifications. Laser Excitation. 488 nm (rated at 20,000 hr life) 640 nm (rated at 20,000 hr life) Laser Profile. 10 x 75 µm . Laser Power . 14.7 mW 640 nm Diode Red Laser
With 2 lasers: 488 blue and 635 Red, and 4 parameters. The Accuri has no adjustable PMT so there are no voltages to set, making it very straight forward to use.
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An analytical flow cytometer equipped with autosampler, 2 lasers (blue and red), 2 scatter detectors and 4 emission detectors and a range of
Turn on the computer and log in with your UCF info. Start the Accuri CSampler software. Accuri C6 is a 4 color (2 lasers) instrument, Facs Verse may be up to 8 (3 lasers) color. So the choice should be addressed by the kind of flow experiments you plan to do.